Fig. 1: Tail fin transection induces a temporal-spatial activation of NF-kB and TNFα in macrophages migrating to the injury. | npj Regenerative Medicine

Fig. 1: Tail fin transection induces a temporal-spatial activation of NF-kB and TNFα in macrophages migrating to the injury.

From: An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish

Fig. 1

Macrophages in transgenic larvae expressing fms:gal4; UAS:NfsB-mCherry were tracked at the site of tailfin amputation over time (t) in hours post-amputation (hpa) and number of macrophages present at the injury plotted (A, B, n = 7 animals, colours represent individual animals). Macrophages expressing NFKB:EGFP were visualised and quantified at 1, 2, 3, 4 hours post-amputation (C–E, I, n = 3 animals). Macrophages expressing tnfa:egfp were visualised and quantified at 1, 2, 3 and 4 hours post-amputation (F–H, J, n = 6 animals). Bar plots show mean values with bars representing the standard error of the mean. Scale bars 100 µm (A, C, D, E, F, G, H) or 25 µm (C’, D’, F’, G’).

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