Fig. 3: TNFα expression is elevated in macrophages responding to injury when NF-kB signalling is inhibited. | npj Regenerative Medicine

Fig. 3: TNFα expression is elevated in macrophages responding to injury when NF-kB signalling is inhibited.

From: An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish

Fig. 3

Tailfin injuries were performed on transgenic larvae expressing tnfa:egfp reporter and animals exposed to 2.5 mM BMS-345541 (A–F) or 300 µM JSH-23 (G–L). Animals were observed by time-lapsed microscopy and macrophages (magenta) expressing tnfa:egfp (cyan) in the tailfin (magnified region) visualised at 1 or 4 hpa, transformed to positive values and expressed as a ratio. The difference in proportion of macrophages expressing tnfa:egfp reporter between conditions was tested by 1-way ANOVA with Dunnett’s multiple comparison test at each time point (M, N, n = 4-6 animals per condition). Significance for differences between treated and control samples is shown (***p < 0.005, **p < 0.01, *p < 0.05, n.s. not significant). Bar plots show mean values with bars representing the standard error of the mean. Scale bars 100 µm (A–L) or 25 µm (A’, B’, D’, E’, G’, H’, J’, K’).

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