Fig. 4: Macrophage responses to injury are altered in the presence of IKK inhibitors. | npj Regenerative Medicine

Fig. 4: Macrophage responses to injury are altered in the presence of IKK inhibitors.

From: An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish

Fig. 4

Macrophages within 100 µm of the injury were observed by time-lapsed microscopy for 4 hpa after tailfin injury of 3 dpf larvae (A). Macrophage migration was tracked towards the fin fold (outlined) using Imaris (yellow lines) to generate measures of instantaneous speed and directionality (B–D). Injured 3 dpf larvae expressing fms:gal4; UAS:NfsB-mCherry (red) were treated with DMSO (control, B), 2.5 mM BMS-345541 (C) or 300 µM JSH-23 (D) immediately after amputation. Speed (E) or directionality (F) of each cell in animals for each condition was plotted, and the significance of differences between control and drug-treated animals was tested by 1-way ANOVA with Dunnett’s test (n = 6 animals/condition). Significance is shown (***p < 0.005, **p < 0.01, *p < 0.05, n.s. not significant). Bar plots show mean values with bars representing the standard error of the mean. Scale bar 100 µm (A–D).

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