Fig. 5: Macrophage responses to injury are altered in tnfrsf1a^sa8496 mutants.

Macrophage responses in larvae with varying tnfrsf1a genotypes were visualised by time-lapsed microscopy for 4 hpa (A–C). Cell behaviour was quantified using Imaris (yellow lines) and measures of cell directionality (D), instantaneous speed (E), and persistence (F) obtained for mutant (MUT), heterozygotes (HET) and wild-type animals (WT). Macrophage responses to injury were examined in larvae with varying genotypes in the presence of 70 µM PTX or in untreated control larvae and directionality (G) and speed (H) were quantified. Significance of differences between genotypes for directionality and speed was tested by a two-tailed unpaired Student’s t-test (n = 3 animals/genotype). Significance is shown (***p < 0.005, **p < 0.01, *p < 0.05, n.s. not significant). Bar plots show mean values with bars representing the standard error of the mean. Scale bar 100 µm (A–C).