Fig. 6: Macrophage-specific inhibition of NF-kB activity perturbs migration in a TNFa-dependent manner. | npj Regenerative Medicine

Fig. 6: Macrophage-specific inhibition of NF-kB activity perturbs migration in a TNFa-dependent manner.

From: An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish

Fig. 6

Macrophage responses in larvae expressing IkBA-SR in macrophages (B) and control animals without the transgene (A, WT) were visualised by time-lapsed microscopy for 4 hpa. Macrophages were visualised in larvae expressing IkBA-SR in macrophages and injected by Cas9 and gRNA to tnfa (C, +tnfa CRISPR) or in sibling uninjected control larvae (D). Cell behaviour relative to the injury (white line) was quantified using Imaris (yellow lines) and measures of instantaneous speed (E, G), and directionality (F, H) compared between conditions. Significance of difference between conditions for speed and directionality was tested by two-tailed unpaired Student’s t-test (WT vs fms:gal4; UAS:IkBA-SR n = 3 animals/condition, fms:gal4; UAS:IkBA-SR vs fms:gal4; UAS:IkBA-SR + tnfa gRNA/Cas9 n = 6 animals/condition). Significance is shown (***p < 0.005, **p < 0.01, *p < 0.05, n.s. not significant). Bar plots show mean values with bars representing the standard error of the mean. Scale bar 100 µm (A–D).

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