Fig. 1: Off-target cell population develops in iPSC-derived kidney organoids and disrupts the renal structures’ development.
From: FGF9 treatment reduces off-target chondrocytes from iPSC-derived kidney organoids
A Schematic of kidney organoid culture. iPSCs were stimulated with CHIR99021 for 3 days and a FGF9/heparin cocktail for 4 days. After a 1 h pulse of CHIR99021, cells were aggregated and cultured at the air–liquid interface for 5 days in the presence of the FGF9/heparin cocktail. Organoids were then cultured until day 7 + 25 without growth factors. A spherical kidney organoid shape was observed at day 7 + 18 B and 7 + 25 (C) by brightfield microscopy. Renal structures assessed by immunofluorescence in cryosections show D, F glomeruli (NPHS1), proximal tubules (LTL), distal tubules (ECAD); E, G loops of Henlé (SLC12A1), and a stromal population (MEIS1/2/3). Nuclei are stained with DAPI. Asterisks indicate an off-target cell population at day 7 + 25 (F, G). Scale bars represent 1000 µm (B, C) and 50 µm (D–G).
