Fig. 4: FGF9 treatment reduces expression of cartilage markers at day 7 + 25.
From: FGF9 treatment reduces off-target chondrocytes from iPSC-derived kidney organoids
A–E FGF9 treatment significantly decreased five markers of chondrogenesis in kidney organoids at day 7 + 25 compared to control organoids. Gene expression was assessed by qPCR and shown as -fold change compared to expression at day 7 + 18. **p < 0.01; ***p < 0.001; N = 5. F Western blotting of SOX9 and COL2A1 protein levels in kidney organoids at day 7 + 25 showed decreased expression in FGF9-treated organoids compared to untreated controls. GADPH levels are shown as loading controls. Quantification of protein levels confirmed significantly decreased expression of SOX9 (G) and COL2A1 H at day 7 + 25 with FGF9 treatment (+) compared to nontreated (−) organoids. **p < 0.01 from 3 to 4 samples. Relative expression was assessed as the -fold change compared to day 7 + 18.
