Fig. 1: Fibrillar collagen deposition in regenerative and fibrotic mouse hearts at 4-days post-injury. | npj Regenerative Medicine

Fig. 1: Fibrillar collagen deposition in regenerative and fibrotic mouse hearts at 4-days post-injury.

From: Type V collagen from macrophages regulates initial collagen assembly and alignment in post-infarcted hearts

Fig. 1

Confocal imaging of an immunofluorescent-stained cross-section from P1 or P7 hearts at day 4 post-MI (4dpi) showing endomucin (EMCN)-stained vessels (red) and Collagen I (Col I, white; a, a’ and d, d’). Collagen III (Col III, white; b, b’ and e, e’) and Collagen V (Col V, white; c, c’ and f, f’). a’-f’ are magnified views of the inset boxes in (a–f). LV: left ventricle. Epi: epicardium. Representative images of n = 3, scale bar: 100 μm. Transmission electron microscopy (TEM) of the scar region of a P1 heart at 4dpi showing the injured area and cardiomyocytes (g). A fibroblast and surrounding collagen fibrils (white arrows) in the same P1 heart at 4dpi (h). Magnified collagen fibrils (i). Scar area of a P7 heart 4dpi showing dying cardiomyocytes, fibroblasts and collagen (j). A fibroblast and collagen fibrils (k). Magnified collagen fibrils in the P7 heart 4dpi (l). Scale bar as indicated. M: cardiomyocyte. Fb: fibroblast; Nu: neutrophil; ER: endoplasmic reticulum. Cl: collagen.

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