Fig. 3: Fibrillar collagen deposition in regenerative and fibrotic mouse hearts at 21 days post-injury. | npj Regenerative Medicine

Fig. 3: Fibrillar collagen deposition in regenerative and fibrotic mouse hearts at 21 days post-injury.

From: Type V collagen from macrophages regulates initial collagen assembly and alignment in post-infarcted hearts

Fig. 3

Confocal imaging of an immunofluorescent-stained cross-section from P1 or P7 hearts at day 21 post-MI (21dpi) showing endomucin (EMCN)-stained vessels (red) and Collagen I (Col I, white; a, a’, d, d’:). Collagen III (Col III, white; b, b’, e, e’) and Collagen V (Col V, white; c, c’, f, f’). a’-f’ are magnified views of the inset boxes in (a–f). LV: left ventricle. Epi: epicardium. Representative images of n = 3, scale bar: 100 μm. TEM of myocardium proximal to suture of a P1 heart at 21dpi showing cardiomyocytes and collagen fibrils (g). Magnified view of the inset box in (g) showing the cardiomyocyte and the collagen fibrils (h). Magnified view of collagen fibrils in the same section (i). TEM of the scar area of a P7 heart at 21dpi showing cardiomyocytes, fibroblasts and collagen fibrils (j). Magnified view from the inset box of (j) showing abundant collagen fibrils (k). Magnified view of collagen fibrils in the same section (l). Scale bar as indicated. CM: cardiomyocyte. Cl: collagen. FB: fibroblast.

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