Fig. 6: Col V deletion from macrophages results in altered scarring and compromised collagen alignment. | npj Regenerative Medicine

Fig. 6: Col V deletion from macrophages results in altered scarring and compromised collagen alignment.

From: Type V collagen from macrophages regulates initial collagen assembly and alignment in post-infarcted hearts

Fig. 6

Schematic showing the Col5a1 deletion strategy in CD68+ macrophage lineages. Col5a1fl/fl, hCD68-CreERT2/+ males were crossed with Col5a1fl/fl, tdTomatoAi14/tdTomatoAi14 females. Progenies were administered 200 mg tamoxifen for 5 days and then subjected to LAD ligation surgery and analysed at the following timepoints (a). Masson-Trichrome staining of serial sections from three controls and three mKO samples showing the dilated and thinned left ventricles (b). Scale bars: 5 mm. Magnified Masson-Trichrome staining of representative sections from a control (CON) and an mKO heart (c, upper panel). Polarized laser microscopy on picrosirius red staining (lower panel) of serial sections from the same control and mKO hearts showing the collagen fibrils (red) in the left ventricle myocardial wall (c, lower panel). Scale bars 10 μm. Degree of anisotropy analysed with Fibriltool based on polarized laser microscopy on picrosirius red staining of serial sections from the control (CON) hearts and mKO hearts (d) N = 3 per group; p = 0.0082. Ejection fraction (EF, e), Fractional area change (FAC, f) and end diastolic (g) and systolic volumes (h) as determined by echocardiography (ECHO) comparing controls and Col5a1mKO mice at baseline nad different time points post-MI. N = 4 per group; EF, baseline p = 0.78, 7dpi p = 0.32, 14dpi p = 0.31, 21 dpi p = 0.24; FAC, baseline p = 0.60, 7dpi p = 0.51, 14dpi p = 0.22, 21 dpi =0.09; EDV, baseline p = 0.94 7dpi p = 0.23, 14dpi p = 0.41, 21 dpi =0.47; ESV, baseline p = 0.96, 7dpi p = 0.24, 14dpi p = 0.34, 21 dpi =0.19.

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