Fig. 7: Ultrastructure defects in the extracellular matrix (ECM) of macrophage-Col V depleted mice. | npj Regenerative Medicine

Fig. 7: Ultrastructure defects in the extracellular matrix (ECM) of macrophage-Col V depleted mice.

From: Type V collagen from macrophages regulates initial collagen assembly and alignment in post-infarcted hearts

Fig. 7

TEM showing the morphology of cells and associated collagen fibrils in infarcted hearts of Col5a1fl/fl (CON) and Col5a1fl/fl, CD68-CreERT2+ (mKO) mice at 4dpi (a–l), 7dpi (M-S) and 21dpi (T-Z). Lower magnification views of serial sections of the injured area within the myocardium of a CON (a) and mKO heart at 4dpi (d). Collagen fibril bundles in the myocardium of CON (b, c) and mKO hearts (e, f). Macrophages in close proximity to collagen fibrils in CON (g) and mKO hearts (j) at lower magnified views highlighting differences in collagen fibril bundle formation, deposition and alignment (h, i, k, l). Lower magnification views of serial sections of the injured area within the myocardium of a CON (m) and mKO heart at 7dpi (q) with higher magnification insets (n) and (r). Higher magnification to show differences in collagen fibril bundles and fibres in the myocardium of CON (o, p) and mKO hearts (s). Macrophages proximal to collagen fibrils in CON (N) and mKO hearts (R). Lower magnification views of serial sections of the injured area within the myocardium of a CON (t) and mKO heart at 21dpi (X). Magnified view from (t) showing collagen fibrils associated with a fibroblast (u) and collagen fibrils in transverse section (v) and longitudinal sections (w) in CON hearts. Magnified view from (x) showing collagen fibrils and a fibroblast (y) and disorganised collagen fibrils in transverse section in the mKO heart (z). Scale bar: as indicated. CM: cardiomyocyte. Cl: collagen. M: macrophage. FB: fibroblast.

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