Fig. 1
From: Genetic interaction of DISC1 and Neurexin in the development of fruit fly glutamatergic synapses
Fruit fly NMJs and screening of interacting genes. a Schematic presentation and a confocal image of the fruit fly larval NMJs. The larval NMJs exhibit stereotypic synaptic connections between the identifiable presynaptic motoneuron and the specific postsynaptic muscles. Each of the presynaptic boutons made on the target muscle is surrounded by an intricately convoluted post-synaptic membrane structure called subsynaptic reticulum (SSR), which contains scaffolding proteins and postsynaptic signaling complexes. b Screening of interacting genes. Mutant flies (+/CyO-GFP; mutation/TM6B-GFP) of the fruit fly homologue for a psychiatric risk factor gene are crossed with the control (+/+; tubP-GAL4/TM6B-GFP) or the DISC1 OE (UAS-DISC1; tubP-GAL4/TM6B-GFP) flies. The phenotypes of the larval NMJs between the control (+/+; mutation/tubP-GAL4) and DISC1 OE (+/UAS-DISC1; mutation/tubP-GAL4) animals were compared
