Fig. 3

Expression of presynaptic and postsynaptic proteins in NMJ boutons. a–d Active zone formation with or without DISC1 overexpression. Representative confocal images. a, b w (CS10) control animals. c, d dnrx1 ^d08766/+ heterozygotes. Larval NMJs were immunostained with anti-HRP (green) and anti-BRP (magenta) antibodies. Scale bar, 20 μm. e Quantification of BRP expression level in the muscle 6/7 boutons normalized to HRP immunoreactivity. f Quantification of active zone density as determined by the number of BRP puncta per bouton area. g–j Expression of DGLURIIA with or without DISC1 overexpression. Representative confocal images. g, h w (CS10) control animals. i, j dnrx1 ^d08766/+ heterozygotes. Larval NMJs were immunostained with anti-HRP (green) and anti-DGLURIIA (magenta) antibodies. Scale bar, 20 μm. k–n Expression of DLG with or without DISC1 overexpression. Representative confocal images. k, l w (CS10) control animals. m, n dnrx1 ^d08766/+ heterozygotes. Larval NMJs were immunostained with anti-HRP (green) and anti-DLG (magenta) antibodies. Scale bar, 20 μm. o Quantification of DGLURIIA expression level in the muscle 6/7 boutons normalized to HRP immunoreactivity. p Quantification of DLG expression level in the muscle 6/7 boutons normalized to HRP immunoreactivity. Data are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 by one-way ANOVA followed by the Tukey’s pot hoc test. Number of each sample is indicated at the bottom of the bar. The statistical values are listed in Supplementary Table 1