Fig. 5
From: Genetic interaction of DISC1 and Neurexin in the development of fruit fly glutamatergic synapses
Suppression of DNRX1 with DISC1. a–f Representative confocal images of wild-type NMJs with or without DISC1 overexpression. a, b Ubiquitous expression with tubP-GAL4. c, d Pre-synaptic expression with elav-GAL4. e, f Post-synaptic expression with C57-GAL4. a, c, e Control NMJs without DISC1 expression. b, d, f NMJs with DISC1 overexpression driven by the designated GAL4 driver. Synaptic boutons at the NMJs on the muscle 6/7 in the second abdominal segment were immunostained with anti-HRP (green) and anti-DNRX1 (magenta) antibodies. Scale bar, 20 μm. g Quantification of DNRX1 expression level in the muscle 6/7 boutons normalized to HRP immunoreactivity. h Quantification of HRP immunoreactivity in the muscle 6/7 boutons. Data are means ± SEM. Data are means ± SEM. *p < 0.05, **p < 0.01 by one-way ANOVA followed by the Tukey’s pot hoc test. Number of each sample is indicated at the bottom of the bar. The statistical values are listed in Supplementary Table 1
