Fig. 2

mRNA expression levels of synapse refinement receptors ITGAM (a), TREM2 (b) and P2RX7 (c) in mo-MΦs was compared between a non-stimulated state and after pro-inflammatory (n = 30) or anti-inflammatory (n = 29) phenotype induction. The pro-inflammatory phenotype was induced by 6-h stimulation with LPS or R848 and the anti-inflammatory phenotype was induced by 72-h stimulation with IL-4 or dexamethasone. ITGAM (a) and TREM2 (b) mRNA levels were significantly decreased after LPS (p < 0.01) and R848 (p < 0.01), increased after IL-4 (p < 0.01) and not altered after dexamethasone stimulation. P2RX7 (c) mRNA levels were not altered after dexamethasone stimulation, but significantly decreased after IL-4 (p < 0.01) and increased after LPS (p < 0.01) and R848 stimulation (p < 0.01). We did not distinguish between the schizophrenia patients () and healthy controls () in the statistical analyses. One patient sample gave an undetermined value for P2RX7 expression after dexamethasone stimulation. mRNA expression was analyzed with qPCR. All values were normalized (2^Δct) with GAPDH as the reference gene after which the value was multiplied by 1000 to ease visualization. Friedman’s ANOVA tests were used to compare the pro-inflammatory or anti-inflammatory responses to the non-stimulated state. (*p < 0.05; **p < 0.01)