Fig. 3 | npj Schizophrenia

Fig. 3

From: Schizophrenia-related dysbindin-1 gene is required for innate immune response and homeostasis in the developing subventricular zone

Fig. 3

The SVZ of Sdy mice contain more microglia and exhibit increased leucocyte infiltration following peripheral polyI:C administration. a Experimental design showing the time-points of postnatal saline or polyI:C injections in WT and Sdy mice, SVZ samples were collected for analysis at P12. b, c Immunohistochemistry for Iba1 revealed a higher density of immunopositive cells in the SVZ (outlined), corpus callosum (cc) and striatum (str) of Sdy polyI:C compared to control WT saline group. d Sdy mice at P12 had significantly more Iba1+ cells in the SVZ regardless of treatment. e, f Double immunolabelling for Iba1 and CD45 in WT saline and Sdy polyI:C mice. Orthogonal views show a CD45-/Iba1+ cell in the SVZ of a WT saline mouse and a CD45+/Iba1− cell in the SVZ of a Sdy polyI:C mouse. g The total number of CD45+/Iba1− cells in the SVZ at P12 was increased in Sdy polyI:C compared to WT saline mice. All values are mean ± s.e.m. of N = 4 per WT groups and N = 6 per Sdy groups. Data were analyzed using two-way ANOVA for (d) with Tukey’s test for (g). *P < 0.05 and ***P < 0.001. Scale bars 100 μm for (b, c) and 25 μm for (e, f)

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