Fig. 4

Reduced postnatal and adult SVZ proliferation in Sdy polyI:C mice. a Postnatal WT and Sdy mice were injected with saline or polyI:C from P5-9 and brains were collected for analysis at postnatal age P12 or adult P85. b, c Representative images of double-labelled PHi3+/Dcx+ mitotic neuroblasts in the SVZ of WT saline group compared to Sdy polyI:C group. b’,c’ High-magnification confocal z stacks showing boxed areas in b and c with orthogonal views of a PHi3+/Dcx+ cell in b’ and a PHi3+/Dcx− cell in c’. d The mean number of PHi3+ cells was significantly reduced in Sdy polyI:C group in comparison to the WT saline group. e PolyI:C treatment had a significant main effect on the number of PHi3+/Dcx+ mitotic neuroblasts in the SVZ at P12. f, g Representative images showing PHi3+ cells in the SVZ of adult WT saline and Sdy polyI:C mice, with higher magnification of yellow-boxed areas illustrated in insets. h The number of proliferative (PHi3+) cells in the adult SVZ of Sdy polyI:C mice was significantly decreased compared to WT saline mice. All values are mean ± s.e.m. of N = 3 per group for (d, e) and N = 6 per group for (h). Data were analyzed using two-way ANOVA for (e) with Tukey’s post hoc test for (d, h). *P < 0.05. Scale bars 100 μm for (b, c and f, g), 20 μm for (b’, c’) and 15 μm for (f’, g’)