Fig. 2

Neat1 is expressed in OLGs and its knockout affects forebrain OLG-lineage cells. a Bright-field images of Neat1 and Sox10 ISH in murine striatum. Inserts from the frame: Fluorescent ISH for Neat1 and Sox10. Cell nuclei were counterstained with DAPI. The scale bars, 10 µm. Lower panel: Neat1^-/- mice show depleted Neat1 expression. b Fluorescent ISH for NEAT1 and SOX10 in human cingulate cortex (BA32). OLGs nuclei colocalization of NEAT1 and SOX10 is marked by arrowheads. Scale bars are 20 (upper) and 10 (lower) µm. c Flow cytometry scatter plots and analysis of Olig2⁺ and NeuN⁺ labeled cells from forebrains of controls, Neat1^-/-, and Qk^e5 mice. Data are expressed as mean ± SEM (N = 5/group). ***Student’s two-tailed t-test; p < 0.001