Fig. 4
Neat1 expression is associated with OLG-specific markers. a Volcano plot of mRNA expression in frontal cortex of NEAT1-/- compared with controls. The OLG-specific66 DEGs are highlighted (red) and labeled (yellow). b Heatmap showing gene expression of affected genes influencing OLG differentiation in Neat1-/- and SZ. Scale indicates expression relative to their appropriate controls; t-scores and p-values are shown in Table 1. c Ratio plots (fold change ± SEM) of mRNA levels of OLG-specific genes, in the frontal cortex and white matter of Qkie5 and Neat1-/- mice as determined by qPCR analyses (n = 6/group for Qkie5 and n = 5/group for Neat1-/-). Insert plot shows ratios for Pspc1 and Neat1 in Qkie5 mice. d Immunofluorescence staining of Qki-5 in the forebrain of controls and Neat1-/- mice. Cell nuclei were counterstained with DAPI. Scale bar, 10 µm. Immunostaining was repeated on nine sections from two independent animals from each experimental group. Fluorescence measurements in outlined DAPI-stained nuclei were performed using ImageJ 1.50b software (Wayne Rasband, NIH, USA) on six adjacent sections for each animal. Data are expressed as mean ± SEM. ***Student’s two-tailed t-test; p < 0.001; **p < 0.01; *p < 0.05
