Fig. 3
From: Muscle tissue engineering in fibrous gelatin: implications for meat analogs
Fibrous gelatin and its preparation for tissue culture. a Microfibrous gelatin produced by immersion rotary jet spinning and cut into samples with ~1.5 mm thickness and 6 cm2 area were plated individually, seeded with cells and cultured with manufacturer-supplied cell culture media. Scaffolds had multiscale fibrous architectures; scale bars are 1 cm. b Rabbit skeletal muscle myoblast cell (RbSkMC) culture and tissue formation depended on gelatin fiber length and crosslinking conditions. We observed (i) spherical aggregates promoted by short-length (~20 μm) fibers, (ii) structurally weak slurry-like tissues resulting from RbSkMC culture in gelatin fibers that were partially crosslinked enzymatically, or (iii) structurally stable tissues resulting from RbSkMC cultured in chemically crosslinked gelatin fibers; cell nuclei are white (DAPI) and the cytoskeleton (F-actin) is red; scale bars are 20 μm
