Fig. 3: Species level classification of MinION and NextSeq sequenced environmental samples. | npj Science of Food

Fig. 3: Species level classification of MinION and NextSeq sequenced environmental samples.

From: Microbiome-based environmental monitoring of a dairy processing facility highlights the challenges associated with low microbial-load samples

Fig. 3

Taxonomic assignment of MinION and NextSeq sequenced samples generated following the use of different pre-processing and sequencing methods. Pre-processing methods include MDA amplification, no pre-processing (NPP), and spread plating on BHI before washing colonies, pelleting, and treating as a metagenomic sample (Plate). Species level classification was performed using LAST (for MinION) and Diamond (for NextSeq) alignment of reads against the NR database and classification with MEGAN (LR for MinION). Species present in at least 5% in at least one sample are shown.

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