Fig. 5: Genus level classification of environmental samples and controls following different pre-processing methods and sequencing methods.

MEGAN LCA based genera level classification of MinION and NextSeq sequences. Also depicted are Sanger results to genus level for morphologically different colonies from each sample (TBC) along with thermophilic sporeformer enriched (ST) and mesophilic sporeformer enriched (SM) counts. Also included are CFU/swab counts for each culturing type. Sanger results represent relative abundance of a subset of morphologically distinct isolates rather than total isolates.