Fig. 2: Effects of different dietary SeNPs supplementation on the intestinal barrier in mice exposed to diquat.

a Survival rate of mice over a 12-h period during diquat treatment. b–d Biomarkers related to intestinal barrier function damage including FITC-dextran (b) (n = 6), DAO (c) (n = 8), D-LA (d) (n = 8). e The expression levels of tight junction proteins (ZO-1, occludin, claudin 1) detected by Western blot analysis (n = 3). f Jejunum morphology was observed by H&E staining (n = 4). g Goblet cells in the jejunum were observed by AB-PAS staining. h mRNA expression levels of MUC2 in the jejunum (n = 6). i mRNA expression levels of REG3G in the jejunum (n = 6). Data are expressed as the fold change versus the 0.3-Se group or 0.3-Se + diquat group (set to 1). Data are expressed as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.