Fig. 1: Schematic representation of the experimental procedure. Bioengineered intestinal tubules were exposed to crude extracts from Lactuca for 24 h in the cell cultivation medium.
From: Bioengineered intestinal tubules as a tool to test intestinal biological efficacy of lettuce species
The supernatant was collected to assess interleukin (IL)-6, IL-8, and nitric oxide (NO) content. The epithelial integrity was determined after removing the supernatant using the inulin-FITC leakage test. After removing the hollow fiber membrane from the chamber, the tubule was assessed for epithelial integrity via immunofluorescent staining or alkaline phosphatase activity and cell viability. Created with BioRender, license 6221c9168684e9004c47503b.
