Fig. 4: Relative expression of differentiation markers PAX7, MYOD1, MYOG, MYH2 for C2C12 at four different sampling time points on cytodex and mycelium carriers.

Samples were collected right after switching to differentiation media (time point 0, T0) and at 48 hours (time point 1, T1), 96 h (time point 2, T2), and 168 h (time point 3, T3). * indicates significant differences at p ≤ 0.05, ** at p ≤ 0.01, **** at p ≤ 0.0001, with n indicated by individual data points and ns indicating no significant differences. Plate wells that did not amplify are not shown. Error bars represent standard deviation.