Fig. 2: Post-prandial oxidative stress in erythrocytes.
From: Nutritional load in post-prandial oxidative stress and the pathogeneses of diabetes mellitus
The oxidative status in erythrocytes of obese insulin-resistant subjects (blue, n = 7), and lean insulin-sensitive subjects (red, n = 7) challenged with mixed meals, A high fat meal, B high carbohydrates, and C high protein meal. The differentiation between MHL subgroup and MUO subgroup responses to the mixed meal challenges were calculated using receiver operating characteristic analysis. Hierarchical clustering of oxidative stress in erythrocytes levels depicting correlation among 14 subjects in D HF meal, E HC meal, and F HP meal with AUCs evaluated using ROC analysis represented in the form of heatmap. Open-labeled, randomized, cross meal intervention trial was carried out on (i) 7 MUO (27.5 < BMI < 35 kg/m2) insulin-resistant (HOMA-IR > 2.5) subjects and (ii) 7 MHL (20 < BMI < 23 kg/m2) insulin-sensitive (HOMA-IR < 1.6) subjects. High fat, high protein, and high carbohydrates meal were challenged once every 2 weeks, and aliquots of blood were withdrawn at every 30 min interval for 2 h. Redox phenotyping of the erythrocytes was measured by using micro-scale NMR system. Other traditional markers (fasting glucose, insulin, cholesterol etc.) were also recorded.
