Table 1 Translational clinical measurements of oxidative stress in reported case studies61,62,63,64,65,66,67,68,69,70,71,72,73
From: Nutritional load in post-prandial oxidative stress and the pathogeneses of diabetes mellitus
Liquid biopsy | Oxidative stress biomarker | Study design | Methodology | Cohorts | Reference |
|---|---|---|---|---|---|
Blood | DNA damaged in blood leukocytes | Randomized controlled, clinical trial | Act diff 2 hematology analyzer/agilent scanner (G2505C) | 47 female subjects (26 pregnant,21 non-pregnant) | Jiang62 |
Blood | Nitrite and nitrate | Randomized, interventional study | Relevant kit | 24 white rabbits | Madihi63 |
Blood | RBCs | Targeted metabolomics approach | LC/MS analysis | Five male and three female | Fu (2016) |
Blood | Mononuclear cells: Keap-1 protein levels and NQO1 protein levels, and nuclear Nrf-2 DNA binding capacity | Placebo-controlled, crossover intervention study | Not reported | 10 healthy male subjects (aged 37, BMI 22.6) | Ghanim64 |
Plasma | Plasma | Crossover, acute interventional study | spark® multimode microplate reader | 14 healthy subjects (aged 20–30) | Papagianni65 |
Plasma | Plasma, MDA | Randomized, controlled crossover trial | Not reported | 11 healthy male subjects (aged 25.0, BMI 24.7) | Chusak66 |
Plasma | OxLDL | Randomized, double-blind, parallel-groups, placebo-controlled study | Oxidized LDL ELISA kit | 146 healthy normal weight subjects (66 males, 80 females) | Deplanque67 |
Plasma | 8 genes related to oxidative stress: NM_697; NM_2985; NM_14080; NM_1498; NM_581; NM_2574; mithocondrial proton carrier; NM_3355 | Randomized crossover, clinical study | Specific RT2 Profiler PCR Arrays | 22 healthy subjects | De Lorenzo68 |
Plasma | PGF2α | Double-blinded, randomized, placebo-controlled trial | Enzyme-linked immunosorbent assay kits | 51 T2DM patients | Wu69 |
Serum | SOD | Clinical trial | Colorimetric platereader | 17 male subjects (aged 18–35) | Hunter (2023) |
Serum | ROS | Clinical interventional trial | Highly sensitive fluorescent probe | 12 healthy male subjects | Laura70 |
Urine and blood | 8-Hydroxydeoxyguanosine (8-OHdG), isoprostane and hs-CRP | Clinical trial | Enzyme-linked immunosorbent assay kit/enzyme-linked immunosorbent assay kits | 10 T2DM patients (aged 33.9) | Kakuda71 |
Urine | MDA, SOD, CAT, GPx, GSH,GST | Randomized, double-blind, placebo-controlled trial | Enzyme-linked immunosorbent assay (ELISA) kits | 90 healthy subjects | Lee72 |
