Table 1 The independent factors and combination of independent factors for optimization the ultrasound-assisted extraction of astaxanthin with different solvents from F. merguiensis shrimp wastes

From: Green astaxanthin extraction using microwave and ultrasound pretreatments

Run

Independent variables

Total carotenoid content (µg/g DW)

Astaxanthin concentration (mg/mL)

Astaxanthin recovery yield (%)

X1

X2

X3

Organic Solvent

Vegetable oil

Ionic microemulsion

Organic Solvent

Vegetable oil

Ionic microemulsion

Organic Solvent

Vegetable oil

Ionic microemulsion

1

5

200

5

46.936

40.745

76.142

37.539

38.491

69.142

83.33

93.66

91.33

2

20

60

30

28.476

17.682

49.682

21.555

16.205

48.047

78

91

96.33

3

5

130

17.5

57.332

35.015

78.92

45.158

34.11

72.761

81.33

97

91.66

4

20

130

17.5

35.983

18.666

48.46

26.761

16.936

47.11

75.33

92

96.66

5

5

60

30

59.586

43.253

79.777

45.888

41.713

76.301

78.66

96

95.33

6

5

200

30

64.142

45.364

90.459

53.444

43.628

85.475

85.66

96

93.66

7

12.5

130

17.5

53.444

24.047

65.443

37.793

19.634

62.031

71.33

81.33

94

8

12.5

130

17.5

35.047

28.856

74.761

26.285

27.507

72.412

81

94.33

96

9

12.5

60

17.5

52.078

14.65

56.555

43.571

13.968

53.713

83.66

94.33

94.33

10

12.5

130

30

51.31

26.333

66.682

41.793

24.872

63.936

84.66

93.66

95.33

11

20

60

5

31.92

8.682

50.746

25.555

7.364

50.333

80.66

84.66

98.33

12

12.5

130

5

44.412

24.079

65.031

36.618

22.444

62.65

84.66

93.66

96

13

20

200

5

36.745

12.84

44.904

25.793

10.253

41.237

69.66

79

90.66

14

12.5

130

17.5

49.047

31.92

65.65

33.317

30.539

62.269

71

95.66

94

15

12.5

130

17.5

54.444

16.793

64.73

38.872

15.888

62.189

73.33

94

95.33

16

5

60

5

53.618

31.65

78.047

42.73

30.507

73.38

83.66

96

93.66

17

12.5

200

17.5

30.84

27.856

58.523

21.888

26.095

56.301

77

92.33

95.66

18

12.5

130

17.5

57.063

24.015

67.031

41.729

19.221

64.348

74.33

79.66

95.33

19

12.5

130

17.5

53.317

37.945

70.586

39.682

36.603

67.364

77.33

96

94.66

20

20

200

30

45.825

28.729

47.364

33.031

27.221

45.714

72.33

94.33

96

  1. X1, X2, and X3 are independent variables that correspond to the ratio of solvent to sample, power of the device, and extraction time, respectively.