Table 8 The independent factors and combination of independent factors for optimization the microwave-assisted extraction of astaxanthin with different solvents from P. maeoticus Gammarus

From: Green astaxanthin extraction using microwave and ultrasound pretreatments

Run

Independent variables

Total carotenoid content (µg/g DW)

Astaxanthin concentration (mg/mL)

Astaxanthin recovery yield (%)

X1

X2

X3

Organic solvent

Vegetable oil

Ionic microemulsion

Organic solvent

Vegetable oil

Ionic microemulsion

Organic solvent

Vegetable oil

Ionic microemulsion

1

20

300

5

13.897

6.252

28.475

10.936

5.587

27.856

77

89

97.33

2

20

200

57.5

14.983

7.841

32.237

10.444

7.507

31.46

69

95.33

97

3

12.5

200

57.5

20.682

8.777

45.968

15.126

8.317

45.063

73

94

97.33

4

12.5

200

57.5

14.682

6.009

56.475

9.983

4.205

49.301

67

67.66

88

5

20

100

110

12.857

2.968

64.887

8.666

2.444

45.714

67

81

70

6

12.5

200

110

18.401

7.444

68.602

12.777

6.285

66.65

68.66

83

96.66

7

12.5

200

5

13.396

9.986

47.921

8.771

8.663

47.031

65

87.66

97.33

8

5

300

5

16.396

4.12

81.824

14.269

3.317

54.983

86.33

80.66

66.66

9

12.5

200

57.5

15.174

3.079

52.38

11.443

2.523

51.412

74.66

80.66

98

10

5

200

57.5

15.358

7.65

48.713

13.348

6.761

47.825

85.66

86

97.66

11

5

100

5

26.094

5.92

60.459

20.19

5.552

58.406

77

93

96.66

12

12.5

100

57.5

16.396

5.57

54.888

11.825

4.586

53.809

71.66

81.66

97.66

13

12.5

200

57.5

16.983

8.253

74.748

11.571

7.507

71.38

68

88.33

95.33

14

20

300

110

14.539

4.348

60.412

10.079

3.824

47.555

69

81.66

78.66

15

12.5

200

57.5

16.285

10.158

59.031

11.714

7.983

44.951

71.33

75

77.66

16

12.5

300

57.5

16.358

7.444

89.396

11.38

6.65

66.111

69

88.66

72

17

5

300

110

25.729

5.824

54.936

17.555

4.735

52.285

68

81.33

95

18

5

100

110

22.428

7.159

45.586

15.999

6.314

44.253

70.66

89

96.66

19

12.5

200

57.5

16.712

6.935

42.841

11.777

6.507

40.745

69.66

93.33

94.66

20

20

100

5

12.174

4.587

31.317

8.809

4.396

30.634

71.66

93.66

97

  1. X1, X2, and X3 are independent variables that correspond to the ratio of solvent to sample, power of the device, and extraction time, respectively.