Fig. 2: Volcano plots of differential metabolite regulation, and hierarchical clustering analysis heatmaps.

A The horizontal axis represents the fold change in metabolite expression between two groups, and the vertical axis represents the statistical test value for the difference in metabolite expression levels. Each point in the graph represents a specific metabolite, and the size of the point represents the Vip value. The red dots represent significantly upregulated metabolites, the blue dots represent significantly downregulated metabolites, and the gray dots represent non significantly differentially expressed metabolites. The corresponding data can be found in the detailed table of differences. B Each column in the figure represents a sample, each row represents a metabolite, and the color in the figure indicates the relative level of the metabolite in the group of samples; the specific trend of level change can be seen in the number label under the color bar at the bottom right. The left side is the dendrogram of metabolite clustering, the right side is the name of the metabolites, the closer the two metabolite branches are, the closer their abundances are; the upper side is the dendrogram of sample clustering, and the lower side is the name of the samples. The closer the two sample branches are, the closer the abundance of all the metabolites in the two samples are, that is, the closer the trend of the metabolite abundance is.