Table 4 Elution gradient of mobile phase
Time (min) | Flow rate (mL/min) | A (%) | B (%) | |
---|---|---|---|---|
Positive ion mode | 0 | 0.4 | 100 | 0 |
3 | 0.4 | 80 | 20 | |
4.5 | 0.4 | 65 | 35 | |
5 | 0.4 | 0 | 100 | |
6.3 | 0.4 | 0 | 100 | |
6.4 | 0.4 | 100 | 0 | |
8 | 0.4 | 100 | 0 | |
Negative ion mode | 0 | 0.4 | 100 | 0 |
1.5 | 0.4 | 95 | 5 | |
2 | 0.4 | 90 | 10 | |
4.5 | 0.4 | 70 | 30 | |
5 | 0.4 | 0 | 100 | |
6.3 | 0.4 | 0 | 100 | |
6.4 | 0.4 | 100 | 0 | |
8 | 0.4 | 100 | 0 |