Fig. 1: Animal experiment and behavioral tests in the LPS-injection model.

A Experimental animal procedures: from day 0, mice were given three doses of Prunella vulgaris seed oil (PVSO) at low (L, 0.0104 g/kg), medium (M, 0.0208 g/kg) and high (H, 0.0312 g/kg) doses, from day 2 to day 7 in LPS model mice and intervention group mice were injected intraperitoneally with lipopolysaccharide (LPS) and control mice (Con) were injected with saline. Mice in the medium PVSO dose showed significant improvement in (B) Grip strength magnitude in the grip strength test (GST). C The immobility time of the tail suspension test (TST). D Latency time of the rod rotation test (RRT), and E Sucrose preference index of Sucrose preference test (SPT). Data are presented as mean ± SEM, *P < 0.05, **P < 0.01 by One-way ANOVA (n = 6 per group).