Fig. 2: Effects of PVSO on cancer progression in mice with 4T1 breast cancer tumors.
A Experimental animal procedures: on day 0, mice were injected subcutaneously on the right side with 1 × 105 4T1 tumor cells or PBS. From day 1 to day 21, mice were gavaged once daily with Prunella vulgaris seed oil (PVSO) and Ganoderma spore oil (GSO) or drinking water (Tumor). On day 7, mice could palpate the tumor. Mice were behaviorally tested on day 21 and then euthanized. Mice were weighed weekly and tumor volumes were measured on days 0, 3, 6, 9, 12, 15, 18 and 21. B Weekly body weight of mice. C Tumor volume changes over time. Both PVSO and GSO showed improvement. D Size of tumors removed after euthanasia. E Sections of tumor tissue stained with hematoxylin and eosin. The cytoplasm is eosinophilic (indicated by the red arrow), Large areas of necrosis were observed in the cancerous tissue of the PVSO group and GSO group (black box), with most of the necrotic cells and disintegrated stroma fused into a blurred, granular, unstructured red-stained substance (blue arrow). F Ki67-immunofluorescence stained-tumor tissue sections. Data are presented as mean ± SEM, Two-way ANOVA (B, C), otherwise use One-way ANOVA, **P < 0.01, ****P < 0.0001 (n = 8–10 per group).
