Fig. 5
Single-cell response of the mar operon with two unrelated previous exposures to inducer. a Open circles denote the median respond to salicylate corresponding to single-cell experimental data using the YFP reporter system (error bars are standard deviations; representation in arbitrary units AU). Bacteria were initially uninduced (blue) or fully induced (red) before exposure to the different dosages of salicylate. Solid gray line indicates the deterministic input function obtained by simulation, while the solid black line represents the median input function predicted by a stochastic model (dashed lines represent standard deviations over the median; all data and simulations here for a ∆rob system). b Probability distributions in steady state for different salicylate concentrations for cells previously uninduced. c As before when cells were previously fully induced (5 mM salicylate). d Model simulation of the probability distributions in steady state for different salicylate concentrations were also independent of the presence/absence of a preceding induction (no hysteresis)
