Fig. 3 | npj Systems Biology and Applications

Fig. 3

From: Model-based identification of TNFα-induced IKKβ-mediated and IκBα-mediated regulation of NFκB signal transduction as a tool to quantify the impact of drug-induced liver injury compounds

Fig. 3

Primary human hepatocytes data and fit. Experimental data was acquired by quantitative immunoblotting of cytoplasmic lysates of primary human hepatocytes treated with either TNFα alone (10 ng/ml) or with TNFα (10 ng/ml) and diclofenac (500 µM). Data points with 1σ confidence intervals computed from independent donors (n = 3, raw data in Figs. S18–S21) are indicated by triangles and error bars. Only the model parameters specific for DCF treatment, observation-specific scaling and offset parameters and a global time-scale parameter were adjusted to predict the correct IKK, IκBα, and NFκB profiles

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