Fig. 4

Demonstration of biomass objective function (BOF) and uptake-rate objective functions (UOFs)-based predictions versus experimental measurements for for CHO cells during early and late exponential phases. a Schematics of the BOF and UOFs inputs and outputs when applied to CHO genome-scale model; b growth rate predictions via BOF using three cell-line specific models and metabolomics datasets for fed-batch of CHO-DG44-derived cell line,9 for early and late exponential phases; c uptake rate predictions of glucose and 5 non-essential amino acids and measurements obtained via UOFs for these same datasets