Fig. 3

Local Young’s modulus of the cell wall is slightly higher for bud than for mother. a Experimental setup: entrapped haploid S. cerevisiae cells were scanned using atomic force microscopy, whereby at each image position the force response to nano-indentation was measured. b Exemplary approach curve for one pixel along with a Sneddon fit (blue). Obtained spatial information is shown for cell wall elasticity c and height d of a budding mother cell. For further analyses, the mean cell wall elasticities from the least curved region of mother and bud ([M] and [B]) were used, avoiding regions of former budding events, bud-scars. e Mean local E at buds compared to mean local E at mothers for 30 measurements on seven cells (same cells, same color). Dashed line: equal cell wall elasticities of bud and mother