Fig. 2
From: Defining subpopulations of differential drug response to reveal novel target populations
Distinct drug response types after unsupervised segmentation of pharmacological response pattern for targeting MAPK or PI3K-AKT signalling. a MAPK and PI3K–AKT pathways illustrating drugs in purple boxes which were assessed, and their different gene targets in the pathway. Genes in the green boxes are involved in the PI3K–AKT pathway while genes in the blue boxes are involved in the MAPK pathway. b Heatmap illustrating 324 pairwise comparison of responses for 18 different inhibitors targeting the MAPK pathway and 18 different inhibitors targeting the PI3K–AKT pathway. The 20th-percentile of log(IC50) values for each drug was determined based on the distribution of log(IC50) values across all cell lines after SEABED segmentation. Based on these 20th percentile cutoffs, we assessed whether there was over-representation of subpopulations in each quadrant and categorised each drug pair into five categories: (i) no preferential response (grey), (ii) subpopulations sensitive to both MAPK and PI3K–AKT pathway inhibitors (pink), (iii) greater MAPK pathway sensitivity (yellow), (iv) greater PI3K–AKT pathway sensitivity (green), or (v) sensitive to either inhibitor but not both (divergent response, blue)
