Fig. 3: Constraints-based metabolic modeling of Glioblastoma predicts targetable alternate flux distributions in the metabolic pathways of temozolomide resistant cells.

a-d Differential predicted flux spans and probability of flux distribution using monte-carlo sampling (blue–U87MG; red–NSP) for (a) Glycolytic pathway. (b) TCA cycle. (c) Folate metabolism and (d) Malate-aspartate shuttle. e Differential flux spans predicted in silico using flux variability analysis (FVA) for U87MG and NSP associated with reprogrammed pathways (arrows point towards their associated pathways showing predicted probability of flux distribution).