Fig. 3: Binary assessment of colony growth for double mutants in all four sets of crosses.

Figures on the left were derived from MATa progeny. Figures on the right were derived from the sister MATα progeny. MATa parents are organized along the x-axis and MATα parents are organized along the y-axis alphabetically by the gene that was knocked out. Rows or columns shaded light gray indicate positions on the plate that should have been empty, because the parent was never generated. The diagonal in each heat map indicates a cross between two parents in which the same gene was knocked out. These should not result in growth under selection. Red cells indicate unexpected growth and are an indication of the false negative rate. Rows and columns shaded dark gray indicate parents that were never generated or were excluded from the analysis, because at least one-third of the progeny resulting from that parent failed to grow. Duplicates of the same gene/marker combination within the same cross are not shown. Total number of crosses (excluding monogenic) = 7350. a, b Cross 1. c, d Cross 2. e, f Cross 3. g, h Cross 4.