Table 1 List of knockout strains with the corresponding gene and protein annotations.

A2m^{tm1b(NCOM)Mfgc}

A2m (232345)

HOM

Q6GQT1

P01023

Alpha-2-macroglobulin-P

Protease inhibitor with activity against all four classes of proteinases

Female pregnancy; negative regulation of complement activation, lectin pathway; stem cell differentiation

Ahcy^{tm1b(EUCOMM)Hmgu}

Ahcy (269378)

HET

P50247

P23526

Adenosylhomocysteinase

Competitive inhibitor of S-adenosyl-l-methionine-dependent methyl transferase reactions

Chronic inflammatory response to antigenic stimulus; circadian sleep/wake cycle; one-carbon metabolic process; response to nutrient; S-adenosylhomocysteine catabolic process; S-adenosylmethionine cycle

C1QA; Ig heavy chain V region MOPC 47A; SERPINF1

Atp5b^{tm1b(EUCOMM)Hmgu}

Atp5f1b (11947)

HET

P56480

P06576

ATP synthase subunit beta, mitochondrial

Mitochondrial membrane ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane

Angiogenesis; ATP biosynthetic process; ATP metabolic process; cellular response to interleukin-7; lipid metabolic process; mitochondrial ATP synthesis coupled proton transport; negative regulation of cell adhesion involved in substrate-bound cell migration; positive regulation of blood vessel endothelial cell migration; proton transmembrane transport; receptor-mediated endocytosis; regulation of intracellular pH

ITIH1

Atp6v0d1^{tm1b(EUCOMM)Hmgu}

Atp6v0d1 (11972)

HET

P51863

P61421

V-type proton ATPase subunit d 1

Subunit of the integral membrane V0 complex of vacuolar ATPase, which provides most of the energy required for transport processes in the vacuolar system

Brain development; cellular iron ion homeostasis; cellular response to increased oxygen levels; cilium assembly; vacuolar acidification; vacuolar transport

C8a^{tm1b(EUCOMM)Hmgu}

C8a (230558)

HOM

Q8K182

P07357

Complement component C8 alpha chain

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune responses

Complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis

BOGM; CA1; CA2; C8A; C8B; C8G; HBZ; Ig alpha chain C region; ISG15

BPGM; CA1; CA2; HBZ; ISG15

Cdk4^{tm1b(NCOM)Mfgc}

Cdk4 (12567)

HET

P30285

P11802

Cyclin-dependent kinase 4

Serine/threonine-protein kinase component of the protein kinascyclin D-CDK4 (DC) complexes that are involved in regulation of G1 phase of the cell cycle

Adipose tissue development; animal organ regeneration; cell division; cellular response to insulin stimulus; cellular response to interleukin-4; cellular response to ionomycin; cellular response to lipopolysaccharide; cellular response to phorbol 13-acetate 12-myristate; circadian rhythm; G1/S transition of mitotic cell cycle; lens development in camera-type eye; negative regulation of cell cycle arrest; positive regulation of apoptotic process; positive regulation of cell population proliferation; positive regulation of cell size; positive regulation of fibroblast proliferation; positive regulation of G2/M transition of mitotic cell cycle; positive regulation of translation; protein phosphorylation; regulation of cell cycle; regulation of cell population proliferation; regulation of gene expression; regulation of insulin receptor signaling pathway; regulation of lipid biosynthetic process; regulation of lipid catabolic process; regulation of multicellular organism growth; response to drug; response to hyperoxia; response to lead ion; response to organic substance; response to testosterone; response to toxic substance; signal transduction

Dhfr^{tm1b(EUCOMM)Wtsi}

Dhfr (13361)

HET

P00375

P00374

Dihydrofolate reductase

Key enzyme in folate metabolism; involved in thymidylate, glycine, purine, and DNA precursor synthesis

Axon regeneration; dihydrofolate metabolic process; folic acid metabolic process; negative regulation of translation; one-carbon metabolic process; oxidation–reduction process; positive regulation of nitric-oxide synthase activity; regulation of removal of superoxide radicals; response to methotrexate; response to nicotine; tetrahydrobiopterin biosynthetic process; tetrahydrofolate biosynthetic process; tetrahydrofolate metabolic process

Ig heavy chain V region MOPC 47A; SERPINF1; KLKB1

Dync1li1^em1(IMPC)Tcp

Dync1li1 (235661)

HET

Q8R1Q8

Q9Y6G9

Cytoplasmic dynein 1 light intermediate chain 1

Non-catalytic accessory component of the cytoplasmic dynein 1 complex that is thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function

Cell cycle; cell division; cellular response to nerve growth factor stimulus; microtubule-based movement; microtubule cytoskeleton organization; positive regulation of mitotic cell cycle spindle assembly checkpoint; regulation of centrosome cycle

G6pd2^em1(IMPC)Tcp

G6pd2 (14380)

HOM

P97324

P11413

Glucose-6-phosphate 1-dehydrogenase 2

Provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis

Glucose-6-phosphate metabolic process; glucose metabolic process; NADP metabolic process; pentose-phosphate shunt

Galc^{tm1b(KOMP)Wtsi}

Galc (14420)

HET

P54818

P54803

Galactocerebrosidase

Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride

Galactosylceramide catabolic process; myelination

Ig heavy chain V region MOPC 47A

Gnpda1^{tm1b(KOMP)Mbp}

Gnpda1 (26384)

HET

O88958

P46926

Glucosamine-6-phosphate isomerase 1

May trigger calcium oscillations in mammalian eggs

Acrosome reaction; fructose 6-phosphate metabolic process; fructose biosynthetic process; generation of precursor metabolites and energy; glucosamine catabolic process; glucosamine metabolic process; N-acetylglucosamine catabolic process; N-acetylneuraminate catabolic process; UDP-N-acetylglucosamine biosynthetic process

Ig heavy chain V region MOPC 47A

Idh1^{tm1b(EUCOMM)Wtsi}

Idh1 (15926)

HOM

O88844

O75874

Isocitrate dehydrogenase cytoplasmic

Catalyzes the reversible oxidative decarboxylation of isocitrate to yield α-ketoglutarate (α-KG) as part of the Krebs cycle

2-Oxoglutarate metabolic process; female gonad development; glutathione metabolic process; glyoxylate cycle; isocitrate metabolic process; NADP metabolic process; regulation of phospholipid biosynthetic process; regulation of phospholipid catabolic process; response to organic cyclic compound; response to oxidative stress; response to steroid hormone; tricarboxylic acid cycle

Iqgap1^{tm1b(EUCOMM)Wtsi}

Iqgap1 (29875)

HOM

Q9JKF1

P46940

Ras GTPase-activating-like protein IQGAP1

Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton

Cell migration; cellular response to calcium ion; cellular response to epidermal growth factor stimulus; cellular response to fibroblast growth factor stimulus; cellular response to platelet-derived growth factor stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; fibroblast migration; glomerular visceral epithelial cell development; negative regulation of dephosphorylation; neuron projection extension; platelet-derived growth factor receptor signaling pathway; positive regulation of cellular protein localization; positive regulation of dendrite development; positive regulation of focal adhesion assembly; positive regulation of MAPK cascade; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine autophosphorylation; positive regulation of protein kinase activity; positive regulation of vascular associated smooth muscle cell migration; regulation of actin cytoskeleton organization; regulation of cytokine production; regulation of mitotic cell cycle; response to angiotensin

CD5L; IGHG1; Ig gamma-2A chain C region sec; IGKC; Ig kappa chain V-II region 7S3; Ig kappa chain V-V region MOPC 149; IGHM; Jchain; SERPINF1; PSMA5

Lmbrd1^{tm1b(EUCOMM)Hmgu}

Lmbrd1 (68421)

HET

Q8K0B2

Q9NUN5

Probable lysosomal cobalamin transporter

Probable lysosomal cobalamin transporter

Insulin receptor internalization; negative regulation of glucose import; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase B signaling

Mfap4^{tm1b(NCOM)Mfgc}

Mfap4 (76293)

HOM

Q9D1H9

P55083

Microfibril-associated glycoprotein 4

Potentially involved in calcium-dependent cell adhesion or intercellular interactions

Cell adhesion; cellular response to UV-B; complement activation, lectin pathway; elastic fiber assembly; regulation of collagen metabolic process; supramolecular fiber organization; UV protection

MFAP4

Mmachc^{tm1.1(NCOM)Mfgc}

Mmachc (67096)

HET

Q9CZD0

Q9Y4U1

Methylmalonic aciduria and homocystinuria type C protein homolog

Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, and the glutathione-dependent reductive demethylation of methylcobalamin and adenosylcobalamin

Cobalamin metabolic process; demethylation; glutathione metabolic process; oxidation–reduction process

Mvk^em1(IMPC)Tcp

Mvk (17855)

HET

Q9R008

Q03426

Mevalonate kinase

Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis

Cholesterol biosynthetic process; isopentenyl diphosphate biosynthetic process, mevalonate pathway; isoprenoid biosynthetic process; negative regulation of inflammatory response; negative regulation of translation

Nek2^em1(IMPC)Tcp

Nek2 (18005)

HOM

O35942

P51955

Serine/threonine-protein kinase Nek2

Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells

Blastocyst development; cell division; centrosome separation; chromosome segregation; meiotic cell cycle; mitotic sister chromatid segregation; mitotic spindle assembly; negative regulation of centriole–centriole cohesion; negative regulation of DNA binding; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; protein autophosphorylation; protein phosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitotic centrosome separation

Npc2^{tm1e(EUCOMM)Wtsi}

Npc2 (67963)

HET

Q9Z0J0

P61916

NPC intracellular cholesterol transporter 2

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment

Cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; intracellular cholesterol transport; intracellular sterol transport; sterol transport

ACTG1; ENO1; CD97; EEF1A1; Ig heavy chain V region MOPC 47A; SERPINF1; PFN1; SELP; TNC; TALDO1; VCL

ACTG1

Pebp1^em1(IMPC)Tcp

Pebp1 (23980)

HOM

P70296

P30086

Phosphatidylethanolamine-binding protein 1

Serine protease inhibitor with activity against thrombin, neuropsin and chymotrypsin, tissue type plasminogen activator and elastase, and RAF1

Aging; eating behavior; hippocampus development; MAPK cascade; negative regulation of MAPK cascade; negative regulation of protein phosphorylation; positive regulation of acetylcholine metabolic process; positive regulation of cAMP-mediated signaling; positive regulation of mitotic nuclear division; regulation of neurotransmitter levels; regulation of the force of heart contraction; response to activity; response to calcium ion; response to cAMP; response to corticosterone; response to drug; response to electrical stimulus; response to ethanol; response to heat; response to oxidative stress; response to toxic substance; response to wounding; sperm capacitation

FN1

Phyh^{tm1c(EUCOMM)Wtsi}

Phyh (16922)

HOM

O35386

O14832

Phytanoyl-CoA dioxygenase, peroxisomal

Converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA.

2-Oxobutyrate catabolic process; 2-oxoglutarate metabolic process; fatty acid alpha-oxidation; isoprenoid metabolic process; methyl-branched fatty acid metabolic process

Pipox^{tm1b(EUCOMM)Wtsi}

Pipox (19193)

HOM

Q9D826

Q9P0Z9

Peroxisomal sarcosine oxidase

Metabolizes sarcosine, l-pipecolic acid and l-proline

l-lysine catabolic process to acetyl-CoA via l-pipecolate; oxidation–reduction process; tetrahydrofolate metabolic process

C1QA; KLKB1

Plk1^{tm1b(EUCOMM)Hmgu}

Plk1 (18817)

HET

Q07832

P53350

Serine/threonine-protein kinase PLK1

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle

Centrosome cycle; establishment of mitotic spindle orientation; establishment of protein localization; female meiosis chromosome segregation; G2/M transition of mitotic cell cycle; homologous chromosome segregation; microtubule bundle formation; mitotic cell cycle; mitotic cytokinesis; mitotic sister chromatid segregation; mitotic spindle assembly checkpoint; negative regulation of apoptotic process; negative regulation of cyclin-dependent protein serine/threonine kinase activity; negative regulation of transcription by RNA polymerase II; nuclear envelope disassembly; peptidyl-serine phosphorylation; polar body extrusion after meiotic divisions; positive regulation of peptidyl-threonine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of proteolysis; positive regulation of ubiquitin-protein ligase activity; positive regulation of ubiquitin-protein transferase activity; protein destabilization; protein localization to chromatin; protein localization to nuclear envelope; protein localization to organelle; protein phosphorylation; protein ubiquitination; regulation of cytokinesis; regulation of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; regulation of mitotic spindle assembly; regulation of protein binding; regulation of protein localization to cell cortex; signal transduction involved in G2 DNA damage checkpoint; synaptonemal complex disassembly

Pmm2^{tm1b(EUCOMM)Hmgu}

Pmm2 (54128)

HET

Q9Z2M7

O15305

Phosphomannomutase 2

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose.

GDP-mannose biosynthetic process; mannose metabolic process; protein N-linked glycosylation; protein targeting to ER

Ptpn12^{tm1b(NCOM)Mfgc}

Ptpn12 (19248)

HET

P35831

Q05209

Tyrosine-protein phosphatase non-receptor type 12

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades

Cellular response to epidermal growth factor stimulus; peptidyl-tyrosine dephosphorylation; protein dephosphorylation; regulation of epidermal growth factor receptor signaling pathway; tissue regeneration

APOD; Ig heavy chain V region MOPC 47A

Pttg1^{tm1b(EUCOMM)Wtsi}

Pttg1 (30939)

HOM

Q9CQJ7

O95997

Securin

Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair

Cell division; cellular process; chromosome segregation; DNA repair; homologous chromosome segregation; mitotic sister chromatid cohesion; negative regulation of cell population proliferation; negative regulation of mitotic sister chromatid separation; regulation of cell growth

IGHG1; IGHM; ITIH3; PSMA1

Rock1^{tm1b(NCOM)Mfgc}

Rock1 (19877)

HET

P70335

Q13464

Rho-associated protein kinase 1

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity

Actin cytoskeleton organization; actomyosin structure organization; apical constriction; apoptotic process; bleb assembly; cortical actin cytoskeleton organization; cytoskeleton organization; embryonic morphogenesis; I-kappaB kinase/NF-kappaB signaling; leukocyte cell–cell adhesion; leukocyte migration; leukocyte tethering or rolling; membrane to membrane docking; mitotic cytokinesis; mRNA destabilization; myoblast migration; negative regulation of amyloid-beta formation; negative regulation of amyloid precursor protein catabolic process; negative regulation of angiogenesis; negative regulation of bicellular tight junction assembly; negative regulation of myosin-light-chain-phosphatase activity; negative regulation of neuron apoptotic process; negative regulation of protein binding; neuron projection arborization; neuron projection development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of amyloid-beta clearance; positive regulation of autophagy; positive regulation of cardiac muscle hypertrophy; positive regulation of connective tissue replacement; positive regulation of focal adhesion assembly; positive regulation of gene expression; positive regulation of MAPK cascade; protein localization to plasma membrane; protein phosphorylation; regulation of actin cytoskeleton organization; regulation of actin filament-based process; regulation of angiotensin-activated signaling pathway; regulation of blood vessel diameter; regulation of cell junction assembly; regulation of cell migration; regulation of establishment of endothelial barrier; regulation of keratinocyte differentiation; regulation of microtubule cytoskeleton organization; regulation of neuron differentiation; regulation of synaptic vesicle endocytosis; response to angiotensin; response to transforming growth factor beta; Rho protein signal transduction

Sra1^{tm1b(EUCOMM)Hmgu}

Sra1 (24068)

HOM

Q80VJ2

Q9HD15

Steroid receptor RNA activator 1

Functional RNA which acts as a transcriptional coactivator that selectively enhances steroid receptor-mediated transactivation

Apoptotic process; cell differentiation; cellular response to estrogen stimulus; negative regulation of myoblast differentiation; positive regulation of transcription by RNA polymerase II; regulation of apoptotic process; regulation of mitotic cell cycle; regulation of transcription by RNA polymerase II

ENO1; BPGM; CA1; CA2; BLVRB; HBA; HBB-B1; HBZ; Ig heavy chain V region MOPC 47 A; PRDX2; PRDX6; SOD1; ISG15

BPGM; CA1; CA2; BLVRB; HBA; HBB-B1; HBZ; PRDX2; PRDX6; SOD1; ISG15

Ulk3^em2(IMPC)Tcp

Ulk3 (71742)

HOM

Q3U3Q1

Q6PHR2

Serine/threonine-protein kinase ULK3

Serine/threonine-protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy

Autophagy; fibroblast activation; negative regulation of smoothened signaling pathway; positive regulation of smoothened signaling pathway; protein autophosphorylation; smoothened signaling pathway

C1QC; FGL1

Ywhaz^{tm1b(EUCOMM)Hmgu}

Ywhaz (22631)

HET

P63101

P63104

14-3-3 protein zeta/delta

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways

Establishment of Golgi localization; Golgi reassembly; histamine secretion by mast cell; protein phosphorylation; protein targeting; protein targeting to mitochondrion; regulation of cell death; regulation of ERK1 and ERK2 cascade; regulation of synapse maturation; response to drug; signal transduction; synaptic target recognition