Fig. 2: PRIME model advancements.

a Advancements in PRIME over previous methods (PROM and IDREAM) are indicated as (1) incorporation of regulatory influences from EGRIN (regression-based interactions are shown as dotted lines), which increases coverage of the regulatory network, (2) incorporation of the magnitude of regulatory influence of TFs on metabolic genes (β—shown as varying edge thickness) instead of probability (p) and FDR values (c) significantly improved the predictive accuracy of environment-specific gene essentiality. b Number of TFs and genes from PRIME, IDREAM, and PROM. c Number of TF–gene interactions identified using regression-based EGRIN and Protein–DNA (P–D) interactions from ChIP-seq data.