Fig. 1: CTCFL-driven DEGs in OVCAR3 cell line.

a Schematic representation of the workflow followed in this study. RNA-seq data were obtained from OVCAR3 cell lines with CTCFL overexpressed and underexpressed from Salgado-Albarran et al.⁷ and subjected to differential expression analysis. Next, CTCFL BS identification and network enrichment analysis was performed to obtain a candidate set of genes. Finally, the candidate gene list was evaluated in OC-derived tumor samples and normal tissue. b Experimental validation of CTCFL overexpression at the protein level by Western Blot. The upper panel shows the protein abundance obtained by densitometry. BORIS band is 65 kDa. (*) indicate nonspecific bands. c Transcript levels of CTCFL and CTCF in each experimental condition. d Change in gene expression and statistical confidence of CTCFL and CTCF in each experimental condition vs control. e Venn diagram with the number of DEGs found in each and shared between experimental conditions. f Relative gene expression (row z-score of normalized reads) of the 149 DEGs found in both experimental conditions classified by the direction of gene expression change. Gene names in red indicate previously reported CTCFL transcriptional targets^7,31,37,89.