Fig. 2: Identification of CTCFL direct targets and CTCFL-deregulated protein interaction network.
From: CTCFL regulates the PI3K-Akt pathway and it is a target for personalized ovarian cancer therapy
a Schematic representation of the workflow followed for the identification of DEGs with CTCFL BS. Sequences flanking the TSS of DEGs found in both experimental conditions were retrieved and used as input for motif identification with HMMer using the reported CTCFL motifs in Jaspar and Factorbook. Next, genes with a predicted BS and experimentally validated CTCFL and CTCF occupancy (ChIP-seq) were selected. b Visual representation of CTCFL and CTCF occupancy in predicted CTCFL BSs of the genes VEGFA, IL12A, WSCD2, RBM20, TGFB1, MAP1B, and CTCFL. c Protein interaction network enriched with CTCFL-deregulated genes found in vitro. d Functional enrichment analysis of genes in the protein interaction network using KEGG, Reactome, and Wikipathway databases.
