Fig. 2: Proteome and transcriptome rewire at late stages of T cell activation.

a Heatmaps show the expression patterns of commonly quantified mRNA transcripts and proteins in CD4 and CD8 T cells. Yellow: upregulated, blue: downregulated. Row clustering but not column clustering was applied when generating the heatmap. b Venn diagrams showing the overlap between quantified mRNA and protein obtained from transcriptomic and proteomic data. c DE proteins encoded by mRNA differentially expressed at 6 h following activation. The number of the proteins regulated at each time point is shown. FDR < 0.05. The dotted line represents the total number of proteins upregulated (yellow) or downregulated (blue). d Pearson correlation between DE genes and proteins over the entire time course of T-cell activation. Blue to yellow gradient shows low to high correlation values for CD4 and CD8 T cells at each time point. e Scatter graph with four quadrants indicates the distribution and correlation between gene and protein expression changes in both CD4 and CD8 T cells at different time points following activation. Each region lists the percentage of T cells falling in each category. mRNA distribution is represented in the “x” axis and protein distribution in the “y” axis. ‘R’ represents the Pearson correlation coefficient.