Fig. 3: CD4 and CD8 T cells become more divergent following TCR stimulation.

a Stacked bar graphs showing the total number of proteins and mRNA transcripts DE between CD4 and CD8 T cells at each time point. Columns represent transcripts and proteins overexpressed in each T cell subset. b Volcano plots showing proteins mRNA DE between CD4 and CD8 T cells at 0 h. Names of the top 10 overexpressed mRNA and proteins in CD8 and CD4 T cells are indicated. c Expression kinetics of proteins upregulated in CD4 (i) and CD8 (ii) T cells and their corresponding mRNA transcripts. Intensities of each time point are shown as mean and the standard error of the mean (SEM) error bars (n = 3). d Heatmap shows the relationship of protein/mRNA expression between DE CD4 and CD8 T cells over the time course. mRNA and protein commonly quantified between two T cell subsets were used. Average linkage and Pearson distance measurement were used in column clustering. The clusters of mRNA transcripts and proteins are indicated by distinct colors.