Table 1 Pseudomonas aeruginosa, Staphylococcus aureus and consortium model statistics
Metabolic models | P. aeruginosa | S. aureus | Consortium |
|---|---|---|---|
Model statistics | |||
Number of reactions | 1495 | 1454 | 3241 |
Number of metabolites | 1283 | 1338 | 2752 |
Number of genes | 1148 | 866 | 2014 |
Number of external metabolites and exchange reactions | 171 | 239 | 293 |
Number of reactions useable for cross-feeding | / | / | 410 (239 + 171) |
MEMOTE and consistency analysis results | |||
Universally blocked reactions | 637 | 293 | 1031 |
Orphan metabolites (consumed but not produced) | 54 | 39 | 93 |
Dead-end metabolites (produced but not consumed) | 84 | 61 | 145 |
Reactions in stoichiometrically balanced cycles | 253 | 46 | 697 |
Extracellular metabolites without exchange reactions | 29 | 38 | / |
Compression results on CSP medium | |||
CSP medium metabolites | 47 | 56 | 57 |
Blocked reactions on CSP medium | 673 | 485 | 1296 |
Number of compressed reactions | 470 | 505 | 1062 |
Number of metabolites of the compressed network | 252 | 440 | 600 |
Consortium partner metabolites on CSP medium | |||
Number of metabolites secreted within the consortium model | 65 | 73 | / |
Number of metabolites taken up within the consortium model | 48 | 68 | / |
Number of metabolites taken up secreted by the other bacterium | 36 | 47 | / |
Minimal cut sets of size three or fewer | |||
Essential reactions on CSP medium | 183 | 309 | 2 |
Synthetic lethal pairs of reactions | 184 | 282 | over 50 ⋅ 103 |
Synthetic lethal triplets of reactions | 216 | 347 | over 50 ⋅ 103 |
Total number of MCSs of size three or fewer | 583 | 938 | unknown |
After tests for consortium growth recovery | |||
Single-species MCSs nullified by consortium metabolite exchanges | 68 | 199 | / |
Single-species MCSs remaining lethal on the consortium | 515 | 739 | / |
Consortium-level MCSs with enzyme target potential | 65 | ||
