Fig. 3: Simulations from Models A, B, and C during model iterations showing improvements in the fitting of the simulations of extracellular limonene (LIMex) and the pooled metabolites DHAP + GAP (pool of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate) with experimental results.
From: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
A Illustrates the poor fitting of LIMex simulation from Model A with the experimental time-series data. B Displays the metabolic network topology where acetaldehyde (ACTLD) and ethanol (ETH) reside, while C shows the simulated ACTLD and ETH concentrations with time from Model A. D Shows an improved LIMex simulation from Model B while E reflects the poor fitting of DHAP + GAP simulation from Model B. F Displays the improved simulation of DHAP + GAP from Model C with the Entner–Doudoroff (ED) pathway included in the metabolic network. Average values are shown for biological replicates (±1 SD) in (A, D–F).
