Fig. 4: Extracellular limonene (LIMex) simulations of the in silico knockout (KO) of enzymes ALDH-ADH (aldehyde dehydrogenase-alcohol dehydrogenase) and LDH (lactate dehydrogenase), and overpressed HK (hexokinase) from the developed dynamic model with the corresponding experimental LIMex concentrations.
From: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
A Compares the simulations of LIMex from the developed dynamic model (Model C) for ALDH-ADH KO, LDH KO, HK overexpressed, and engineered wild-type EcoCTs03, while B shows the LIMex concentrations produced experimentally from the bacterial strains over time. Average values are shown for biological replicates ( ± 1 SD) in (B).
