Table 1 Bacterial strains used in this study
From: Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli
Name | Description | Reference | Remarks |
|---|---|---|---|
E. coli DH5a (ECOS 101) | endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk + ) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F– | Yeastern Biotech | Used for cloning. |
E. coli K-12 MG1655 | F- lambda- ilvG- rfb-50 rph-1 | ATCC 700926 | Used for limonene production. |
EcoCTs03 | E. coli K-12 MG1655 strain transformed with pJBEI-6409 | This study | Strain for limonene production. |
HK overexpressed | E. coli K-12 MG1655 strain transformed with pJBEI-6409 and EcoCTs-CPMS5-glk | This study | Strain for limonene production with glucokinase overexpression. |
E. coli K-12 MG1655 ΔldhA | E. coli K-12 MG1655 with deletion of ldhA gene | This study | Used for limonene production. |
E. coli K-12 MG1655 ΔadhE | E. coli K-12 MG1655 with deletion of adhE gene | This study | Used for limonene production. |
LDH KO | E. coli K-12 MG1655 ΔldhA strain transformed with pJBEI-6409 | This study | Strain for limonene production. |
ALDH-ADH KO | E. coli K-12 MG1655 ΔadhE strain transformed with pJBEI-6409 | This study | Strain for limonene production. |
